, Е.А. Efremov1
, P.А. Elistratov2
, I.А. Yamskov2
, А.F. Askerov2
, V.P. Yamskova3
, V.B. Turovetsky4
N.А. Lopatkin Scientific Research Institute of Urology and Interventional Radiology – branch of the National Medical Research
Radiologiсal Center, Ministry of Health of Russia; Build. 1, 51 3rd Parkovaya St., Moscow 105425, Russia 2
A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences; 28 Vavilova St., Moscow 119991, Russia; 3
N.K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences; 26 Vavilova St., Moscow 119334, Russia; 4
Faculty of Biology of Lomonosov Moscow State University; Build. 12, 1 Leninskie Gory, Moscow 119234
The study objective is to examine the changes in motility of human spermatozoa under the effect of a protein-peptide complex extracted from
cattle testes (Bos taurus).
Materials and methods. The protein-peptide complex was extracted from testes tissue obtained during butchering of cattle at meat process-
ing plants of the Moscow Region. Human spermatozoa were obtained from the ejaculate in the usual way. From the ejaculate sample, 1 ml
was collected for the experiment and control. The protein-peptide complex was added to a test tube in concentrations of 10–8, 10–9, and
10–12 mg/ml. For every concentration, 10–12 experiments were performed. General and active motility were evaluated after 30 min, 1 and
3 hours of incubation at room temperature.
Results and conclusion. The experiments have shown that in the presence of the protein-peptide complex, spermatozoa motility increased
by 10–30 % relative to the initial value at 30 minutes, and this change persisted for 3 hours of observation. The change depended on the
complex concentration: the largest increase was observed at 10–12 mg/ml. Higher complex concentrations did not have a positive effect
on spermatozoa motility.
Key words: pathozoospermia, protein-peptide complex, spermatozoa, membranotropic homeostatic tissue-specific bioregulators, experiment
For citation: Evdokimov V.V., Efremov Е.А., Elistratov P.А. et al. Changes in human sperm motility characteristics in the presence
of protein-peptide complex. Andrologiya i genital’naya khirurgiya = Andrology and Genital Surgery 2018;19(3):70–74.